LNA-Oligonucleotide Production

specific – robust – stable – sensitive

Locked Nucleic Acids (LNA) are a class of synthetic nucleic acid analogues that contain a “locked” bicyclic sugar moiety. This linkage between the 2’-O- and the 4’-C-position locks the ribose in the 3´-endo conformation (see Figure 1) which leads to the characteristic structure of A-form RNA and forms exclusively A-type duplexes.

Compared to traditional DNA or RNA oligonucleotides, the use of LNA modified oligos exhibit enhanced hybridization affinity towards complementary DNA or RNA.¹⁾ LNA oligos can be synthesized using conventional phosphoramidite-chemistry to increase the T_m by 3-8°C for each substituted nucleotide at simultaneously shorter length compared to traditional DNA or RNA oligonucleotides.²⁾ This helps e.g. to detect shorter or very similar target sequences.

Fig. 1: Structures of DNA, RNA and LNA with its C3′-endo, Northern, A-form conformer

Why use LNA

Applications for LNA^4)-5)

Design Guidelines

Ordering information

¹⁾ Harleen Kaur,Amit Arora, Jesper Wengel, Souvik Maiti, Biochemistry 2006, 45, 7347-7355
²⁾ Singh, S.; Nielsen, P.; Koshkin, A.; Wengel, J. LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition. Chemical Communications, 1998, No. 4, 455-456
³⁾ Wahlestedt, C., Salmi, P., Good, L., Kela, L., Johnsson, T. Hökfelt, T., Broberger, C., Porreca, F., Lai, L., Ren, K., Ossipov, M., Koshkin, A., Jakobsen, N., Skouv, J., Oerum, H., Jacobsen M.H. and Wengel, J. (2000).Potent and nontoxic antisense oligonucleotides containing locked nucleic acids Proc. Natl Acad. Sci. USA. 2000, 97, 5633 – 5638.
⁴⁾ Letertre, C.; Perelle, S.; Dilasser, F.; Arar, K.; Fach, P. Evaluation of the performance of LNA and MGB probes in 5’-nuclease PCR assays. Molecular and cellular probes 2003, 17 (6), 307-311
⁵⁾ Karin E.Lundin, Torben Højland, Bo R.Hansen, Robert Persson, Jesper B. Bramsen, Jørgen Kjems Troels Koch, Jesper Wengel, C.I. Edvard Smith, Advances in Genetics, Volume 82, 2013, Pages 47-107.

RNA

RNA-Oligonucleotide Production
fast – reliable – customized – high quality standards

We can offer you the full range of RNA Oligonucleotides. Standard-RNA, modified, O-Methyl, DNA/RNA Chimera as well as Duplex-RNA.
Our RNA-Production follows our high quality standards and all produced RNA Oligos will be QC´d by Maldi or according to the customer's request.

Ordering-Information

Scales and Yields

Large Scale

Large Scale - Oligonucleotide Production
high quality – competitive service

Let us help you determine the best large-scale solutions for your projects, so you can focus on generating informative results instead of spending time and money on troubleshooting.
Our production capacity and synthesis expertise allow us to manufacture highly complex oligos for in vivo, high-throughput, or commercial applications. If needed, we can supply you with grams of highly pure siRNAs/DsiRNAs, aptamers, antisense oligos, ribozymes, miRNAs, decoy oligos, next generation sequencing products, and qPCR primers and probe sets.

Bulk Synthesis of DNA and RNA Oligos

Services

Turnaround Time